With TriStar² S the multi-technology microplate reader platform has been extended in its modularity, performance and user-friendliness by adding a double monochromator for wavelength selection and TRF reading technology.
- BRET and BRET²
- UV/Vis Absorbance
- Time-resolved fluorescence
- TR-FRET / HTRF®
- Fluorescence Polarization (FP)
- Top and Bottom Reading
The TriStar² S has been developed to support a fully modular approach. Any combination of reading technologies and options can be composed maintaining all possibilities of later upgrades.
The optical system ONE-4-ALL with a new revolutionary design is the first time a single optical system has been employed in a multi-technology reader that matches the performance of individual dedicated optical devices. The revolutionary 3D double monochromator offers full flexibility in wavelength selection.
TriStar² S LB 942 employs a double monochromator for absorbance and fluorescecence excitation. The monochromator is predominately meant to provide entire wavelength flexibility and spectral scanning posibilities in absorbance and fluorescence measurements, yet they can be used for Time-Resolved Fluorescence measurements as well.
While a low f number and a high blocking ensure exceptional performance variable bandwidths and increments of 1 nm illustrate the versatility of the monochromators.
Top and Bottom Reading
TriStar2S LB 942 can read microplates from top and from bottom. The latter is especially appropriate for the detection of adherent cells. Bottom reading is available for luminescence and fluorescence readout. Click hereto learn more about the advantages of bottom reading.
Molecular Interaction assays
TriStar2S LB 942 can handle multiple molecular interaction assays. Besides BRET and FRET assays, the instrument is certified for HTRF® TR-FRET interaction studies. For convenient single-label interaction studies, TriStar2S LB 942 can be ordered with a fluorescence polarisation (FP) option.
Built-in Reagent Injectors
Up to 3 reagent injectors can be set up to dispense multiple activators or detection reagents at any time and with user-adjustable volumes during the measurement.
Berthold Technologies´ injectors are based on the proprietary JET Injection technology. Superior accuracy and precision is combined with excellent mixing performance. Variable injections speeds and "friendly" material enable even injection of live cells (e.g. in Aequorin based GPCR screening).
One of the injector tips is located in reading position enabling the measurement of very fast labels, e.g. Acridinium esters, and to inject activators into a running kinetic.
The ergonomic design of the housing provides front access of the injectors keeping the reagent reservoirs within reach and within eyesight. A through allows keeping the reagent bottles on ice or elevated temperatures – just as required.
Excess fluids from priming and washing procedures are safely discarded via a specially designed wash plate.
A temperature controlled microplate compartment ensures stable conditions whenever temperature sensitive enzymes or cells are in use. Condensation of lidded microplates is reduced.
Front Access of Reagents
The reagent reservoirs can be placed on the front for easy access and monitoring. A smoked see through lid protects the reagents from light. A base trough allows for the use of ice to keep the reagents cool.
In the TriStar² S any of reading technologies can be combined with each other and any of the options added.
|Detection Devices||low-noise photomultiplier tube operated in dual mode, spectral range 280 to 650 nm|
photo diode, spectral range 200 – 1000 nm
|Excitation Sources||Xenon flash lamp|
f number: 2.7 (high light transmission)
|Sample/Plate Formats||6 – 384 well plates|
|Temperature Control||up to 45 °C|
including Peltier cooling of photomultiplier
|Shaking||3 modes, 3 velocities|
|Scanning||up to 10,000 points per well|
|Injection||up to 3 JET injectors|
variable volume 10 – 100 µL
|Kinetics Operation||minimum resolution 0.05 sec|
maximum duration up to 7 days
|Luminescence||<6 amol ATP per well|
<3 zmol Firefly luciferase per well (equals less than 800 molecules)
|Crosstalk||5 x 10-6|
|Fluorescence||<200 amol FITC per well|
|Fluorescence Polarization||<5 mP SD (1nM Fluorescein)|
|TRF||<5 amol Europium per well|
dF high > 835 % (Filter)
dF high > 634% (Monochromator)
|Absorbance||accuracy <1 % deviation (@ 2.5 OD)|
precision <0.3 % CV (@ 2.5 OD)
|Dynamic range||>6 decades (PMT)|
4 decades (photo diode)
|Injectors||accuracy better 98 % over entire volume range|
precision better 98 % over entire volume range
|Dimensions||400 x 470 x 345 mm (w x d x h)|
|OS||Win XP, Win Vista, Win 7|
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