The Accuris QuadCount™ improves on the simple technique of brightfield microscopy by utilizing an LED light source, precision optics, and a high-resolution CMOS detector. A finely tuned, motorized XYZ positioning stage provides for extra fine focusing and ability to capture multiple images.
- Fast, accurate and reproducible results
- Intuitive software and touch-screen interface
- Convenient and cost-effective QuadSlides™
- Count sample volumes up to 3.6ul
- Compatible with most common mammalian cell lines (click for detail)
With count speeds at fast as 20 seconds per sample, the Accuris QuadCount provides many advantages over the tedious and time-consuming manual hemocytometry method. The advanced software eliminates human bias in dead/live determinations, reduces counting errors, saves time, and improves efficiency.
The QuadCount utilizes a unique 4-chambered disposable QuadSlide™. Four samples can be processed in one slide (twice the capacity of standard 2-chambered slides), decreasing consumable costs. Each chamber has a hydrophilic inner surface with an engraved grid pattern. High precision production and molding ensure that each QuadSlide meets the rigorous standards and dimensional tolerances required for optimal counting performance.
3 Count Modes Available to analyze different sample volumes.
Quick Mode: 0.15uL / 20 seconds (1 image frame)
Normal Mode: 0.9 uL / 30 seconds (6 image frames)
Precise Mode: 3.6 uL/ 100 seconds (24 image frames)
The QuadCount’s ability to analyze larger sample volumes/multiple images provides significantly better accuracy.
Advanced Optics and Imaging Technology
The Accuris QuadCount incorporates the highest quality optical and electronic components, such as a 4X objective lens and 5MP CMOS detector, to ensure reliable performance and consistent results.
The precision motorized stage adjusts the position of the lens and sensor assembly in micron increments for automatic focus. Multiple image frames (up to 24) can be captured to increase the volume of sample counted, providing a higher level of accuracy.
Correct focus is crucial for accurate counts of viable cells. The QuadCount’s proprietary autofocus system and image analysis software work in conjunction with the motorized stage to analyze and capture images in the proper focal plane.
Advanced detection algorithms easily manage irregularly shaped and aggregated cells. Often misinterpreted by visual counting, correct analysis of these cells is performed by QuadCount’s software, resulting in more accurate counts. User to user variation is eliminated.
Intuitive User Interface
After images are captured, the cell count parameters are easily adjusted.
- Gating size range
- Aggregation level
- Live/dead definition
Cell population statistics are viewed as a histogram and cell size gating can be adjusted. Choose to view the histogram data for individual or multiple chambers in each QuadSlide.
USB ports are available for connecting a printer, bar code scanner, external keyboard, or flash drive. Detailed PDF reports can be exported to a flash drive and transferred to a PC for saving or printing.