NightOWL LB 983 in vivo Imaging System

Berthold Technologies
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LB 983

The pioneering in vivo Imager

More than 20 years ago Berthold Technologies introduced one of the first in vivo imaging systems its Luminograph. For the first time it enabled to detect low light emission in organisms, opening up the opportunity of monitoring reporter genes in animals and plants in a noninvasive way. This new and exciting technology changed the way of medical research offering the important step of working under physiological conditions in a living animal.

Nowadays Berthold Technologies offers with the NightOWL II a state-of-the-art molecular imaging system for in vivo gene expression with luciferases and fluorescent proteins as well as for the localisation with fluorescent-labelled antibodies.

Sensitivity, versatility and ease-of-use in combination make the NightOWL a superior instrument.

  • deep-cooled CCD camera
  • moving camera for flexible field of view
  • signal intensity is height-corrected
  • multiple accessories for different specimens
  • software controlled interfaces and power sockets
  • ports for outside access

Lowest Noise CCD Camera

To get negiligible background even with long exposure times needed with weak expression levels the LB 983 NightOWL II is equipped with a deeply cooled back-illuminated slow scan CCD camera.

With its high full well capacity for a large dynamic range and midband coating to enhance the quantum efficiency in the spectral range between 440 to 770 nm it ideally matches luciferase and GFP emission wavelenghts.

Moving Camera and Sophisticated Sample Cabinet

The sample compartment is extremly light-tight and has enough space to install special light sources or to insert transilluminators, heating tables and other specimen holders. These devices may even be switched on and off through the software and the built-in sockets.

NightOWL is the first imager with an motor-driven camera inside the cabinet for a seamless change of the filed of view. Thus, the sample size can vary between 35 and 260 mm. Positioning is reproducible with ±100 µm and all intensities are height-corrected.

Constant Fluorescence Excitation and Illumination Devices

The light intensitiy to excite the fluorophores in the sample is kept constant through a special circuitry for reliable and repeatable fluorescent measurements. This allows calibration of the imaging system for each fluorophor.

Different illumination systems such as gooseneck fibre optics, ringlights and dual line optics are available to transmit the excitation light in an ideal way.

Light-Tight Ports

This option can be used to introduce heated or cooled liquids and gases, specific cables or even fiber optics and manipulators into the NightOWL sample compartment without compromising the light-tigthness of the system.

IndiGO software

The in vivo imaging software supports complete hardware control and offers extensive tools for image evaluation. It combines quick acquisitions with the possibility for open and individual settings as well as as GLP compliant user management.

X-Ray Imaging

X-Ray transillumination imaging is available in selected countries.

Some techniques for generating and/or detecting light in biological subjects are patented and may require licences from third parties. Users are advised to independently determine for themselves wheter their activities infringe any valid patent.

Detection Devices1 Mpixel CCD (1024x1024), cooled to - 90°C, Pixel size 13x13 µm2, back lit midband-coated full frame chip; full well capacity 100000e-; Readout noise < 3 e- @ 50KHz; dark noise 0.0003 e-/ pixel/ sec @ -80°C; Spectral range: 350-1050 nm, Quantum efficiency 90% at 620 nm; 16 bit A/D conversion; max. frame rate 2.2 fps
Pixel Binningvariable up to 16 x 16
Lensf 0.95, C-mount, 25 mm; resolution from 102 µm/pixel (FOV 10 x 10 mm2) to 253 µm/pixel (FOV 260 x 260 mm2)
Field of Viewmax. 260 mm x 260 mm, min. 10 mm x 10 mm
Position of Viewtop
Sample Heightmax. 312 mm
Exposure Timesfrom milliseconds to hours
Schedulingminutes to weeks
Excitation Sourcehalogen lamp, 75W, 340-750 nm, software controlled lamp stabilisation
Excitation Devicesdual gooseneck
ring light
dual line illumination
Dark chamberabsolutely light-tight; camera mounted on top or at the side; dual flange for mulitport access; software controlled mains outlet; software controlled I/O ports
Dimensionsinstrument: 12200 x 600 x 400 mm (H x W x D)
OSWin XP, Win Vista, Win 7

NightOWL LB 983 Brochure (LB_983_NC_100_Night_Owl_II_-2008-72dpi.pdf, 1,763 Kb) [Download]

LB983 Accessories (lb983_nightowl_accessories_2008_72dpi_brochure_63gg-4j.pdf, 787 Kb) [Download]