TriStar3 LB 942 Multi-detection Microplate Reader

Berthold Technologies

Walk-away Solution

TheLB 931 Microplate Stacker can be used with the multi-detection readers Mithras and TriStar as well as the microplate fluorometer Twinkle. A barcode reader provides positive plate identification making the system a true walk-away solution for microplate reading. Quoted separately.

LB 931 Microplate Stacker

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LB 942

With TriStar3 the multi-technology microplate reader platform has developed into its third generation setting new standards in modularity, performance, and user-friendliness.

  • Luminescence
  • BRET and BRET²
  • Fluorescence
  • FRET
  • Absorbance

The TriStar² has been developed to support a fully modular approach. Any combination of reading technologies and options can be composed maintaining all possibilities of later upgrades.

The optical system ONE-4-ALL with a new revolutionary design is the first time a single optical system has been employed in a multi-technology reader that matches the performance of individual dedicated optical devices.

Built-in Reagent Injectors

Up to 3 reagent injectors can be set up to dispense multiple activators or detection reagents at any time and with user-adjustable volumes during the measurement.
Berthold Technologies´ injectors are based on the proprietary JET Injection technology. Superior accuracy and precision is combined with excellent mixing performance. Variable injections speeds and "friendly" material enable even injection of live cells (e.g. in Aequorin based GPCR screening).
One of the injector tips is located in reading position enabling the measurement of very fast labels, e.g. Acridinium esters, and to inject activators into a running kinetic.
The ergonomic design of the housing provides front access of the injectors keeping the reagent reservoirs within reach and within eyesight. A through allows keeping the reagent bottles on ice or elevated temperatures – just as required.

Wash Plate

Excess fluids from priming and washing procedures are safely discarded via a specially designed wash plate.

Temperature Control

A temperature controlled microplate compartment ensures stable conditions whenever temperature sensitive enzymes or cells are in use.

Front Access of Reagents

The reagent reservoirs can be placed on the front for easy access and monitoring. A smoked see through lid protects the reagents from light. A base trough allows for the use of ice to keep the reagents cool.

Modularity

In the TriStar² any of the reading technologies can be combined with each other and any of the options added.

Detection Deviceslow-noise photomultiplier tube operated in single photon counting mode, spectral range 340 to 650 nm
photo diode, spectral range 200 – 1000 nm
Excitation Sourceshalogen lamp
Sample/Plate Formats6 – 384 well plates
Terasaki plates
Petri dishes
filter membranes
Temperature Controlup to 42 °C
including Peltier cooling of photomultiplier
Shaking3 modes, 3 velocities
Scanningup to 10,000 points per well
Injectionup to 3 JET injectors
variable volume 10 – 100 µL
variable speed
Kinetics Operationminimum resolution 0.05 sec
maximum duration up to 7 days
Performance
Luminescence <6 amol ATP per well
<3 zmol Firefly luciferase per well (equals less than 800 molecules)
Crosstalk 5 x 10-6
Fluorescence <0.3 fmol FITC per well
Absorbanceaccuracy <2 % deviation (@ 2 OD, 405 nm)
precision <0.6 % CV (@ 2 OD, 405 nm)
Dynamic range>6 decades (PMT)
4 decades (photo diode)
Injectorsaccuracy better 98 % over entire volume range
precision better 98 % over entire volume range
Miscellaneous
Dimensions 390 x 470 x 345 mm (w x d x h)
incl. injectors
OSWin XP, Win Vista, Win 7
Regulations CE


LB 942 Brochure (lb942_tristar2_tristar2s_brochure_2015_10_01.pdf, 4,344 Kb) [Download]